Currently approved vaccines consist of live/attenuated microbes or specific protein/polysaccharide subunits of the targeted pathogen capable of inducing protective immunity in the vaccinated patient. The use of mRNA-based vaccines can be advantageous over classical vaccines when combatting certain infectious diseases as they can be quickly adapted to target multiple microbial serotypes, have a favorable safety profile and are cost-effective. In addition, they can be manufactured and deployed rapidly in the face of an emerging infection. The development of efficacious and commercially scalable mRNA vaccines requires overcoming of certain scientific and technical challenges, mainly (1) mRNA molecule instability; (2) inefficient entry and uptake into target cells; (3) impaired antigen translation due to induction of type-I interferons and (4) induction of insufficiently potent immune responses. Herein, we propose the development of a targeted mRNA vaccine delivery system composed of biodegradable lipid particles that ensure effective delivery of intact mRNAs (coding for antigen protein and co-stimulation factor) into immune cells and selective targeting to specific CD8+ dendritic cells using anti-XCR1 scFv to induce potent cellular immune responses. This vaccine platform will be used to prevent pseudomonas aeruginosa infection in a mouse model. This novel mRNA-based vaccine platform could provide a new modality for the prevention of various infectious diseases.
Ph.D. in Biotechnology, Immunology, Molecular Biology or equivalent.
For information on certificates and diplomas issued abroad, please see Degree equivalency
Postdoctoral candidate with experience in the fields of nanotechnology, immunology, infectious disease and/or nucleic acid therapeutics. Previous experience with one or more of the following techniques/models would be considered an asset:
DNA/RNA delivery using nanomaterials
Mammalian cell culture & in vitro screening assays
Generation of plasmid constructs and mRNA vectors
Infectious disease mouse models
Successful candidates should be highly motivated, goal-oriented, able to work independently and simultaneously manage multiple priorities.
Information on language requirements and self-assessment tests
Condition of Employment
Who is eligible?
Fellowships will be for two years contingent on satisfactory progress achieved during the first year.
Candidates should have obtained a PhD (or equivalent) within the past three years (PhD received on or after July 1, 2017) or expect to complete their PhD within 6 months of appointment.
Fellows will work in a lab under the direct supervision of an NRC researcher.
Although preference will be given to Canadian citizens and Permanent Residents, Fellowships are open to nationals of all countries. The offer of a Fellowship does not ensure foreign nationals Permanent Residency in Canada. Non-Canadian candidates should apply for non-immigrant status (i.e. temporary status) and an employment visa. For further information, please contact the closest Canadian High Commission, Embassy or Consulate in your area.
In order to be considered for the program please include the following in your application, please note that you will need to attach the required documents as per the list below when submitting your application. Failure to do so will result in your application being excluded from searches.
Statement of Interest in the project (maximum one page in length)
PhD Transcript - an electronic copy is sufficient, it does not have to be an official version.
List of Publications
When submitting your application you can include the required documents in any attachment field such as «Second language evaluation results» or «Other attachments»
In addition, applicants who best meet the requirements of the position will be asked to provide three letters of recommendation at a later stage of the competition process.
Relocation assistance will be determined in accordance with the NRC's directives.
From $55,541 to $157,009 per annum.
The Postdoctoral Fellowships Program is unique to the NRC. It falls under the RO/RCO classification system which uses a person-based classification system instead of the more common duties-based classification system. Candidates are remunerated based on their expertise, skill, outcomes and impacts of their previous work experience.
NRC employees enjoy a wide-range of benefits including comprehensive health and dental plans, pension and insurance plans, vacation and other leave entitlements.
Fellowships are open to nationals of all countries, although preference will be given to Canadian Citizens and Permanent Residents of Canada. Please include citizenship information in your application.
The incumbent must adhere to safe workplace practices at all times.
Please direct your questions, with the requisition number (9608) to:
Closing Date: 18 March 2020 - 23:59 Eastern Time