Lab Technician

University of Toronto - Toronto, ON (il y a 30 jours)

Postuler dès maintenant

About us:
Home to over 40 departments and institutes, the University of Toronto's Faculty of Medicine lies at the heart of the Toronto Academic Health Science Network and is a global leader in ground-breaking research and education, spanning clinical medicine, basic science and the rehabilitation sciences sectors.

Your opportunity:
The Donnelly Centre for Cellular and Biomolecular Research is an interdisciplinary research institute where scientists make fundamental discoveries in biology to improve health.
Our faculty, staff and trainees come from various fields of science and collaborate to develop and apply cutting-edge tools in genome research, regenerative medicine and computational biology, ultimately to gain new insights about how the human body works. Founded in 2005, the Centre is globally recognized as a leading biomedical research hub thanks to our researchers' landmark discoveries in genetics, stem cells and the molecular basis of disease, as well as state-of-the-art tools in analysis of large-scale datasets.
If you are looking for a fulfilling career in a creative and collaborative environment, this is the place for you.

The Toronto Recombinant Antibody Centre (TRAC) is part of a collaborative project to systematically develop recombinant antibodies to serum-accessible, cancer-associated proteins. As a part of this project, an antibody production and validation laboratory will be established at the Donnelly Centre. As the Lab Technician, you will work collaboratively to identify, produce, and validate the affinity and specificity of novel recombinant antibodies as well as perform general laboratory maintenance duties.

Your responsibilities will include:
Constructing proof of concept experiments to test different elements of the process by conducting numerous molecular- and cell-based experimental approaches to produce high-priority antibodies
Collecting experimental data, verifying results and passing on findings by conducting follow-up experiments, protein expression and purification and enzyme assays; collects experimental data, verifies results by repeating experiments
Providing detailed explanations and demonstrating procedures to students and/or employees on lab techniques, experimental procedures and protocols
Preparing, assembling and setting-up material, equipment and supplies used in teaching and/or research labs
Applying established standards when cleaning lab, rooms and equipment
Troubleshooting issues with lab equipment and/or workstations
Ensuring the safe handling of specialized lab equipment and materials while operating within close proximity to others
Serving as a technical resource on laboratory protocols, and experimental procedures and equipment

Essential Qualifications:
Bachelor's Degree in Biochemistry, Molecular biology or acceptable combination of equivalent experience
Minimum of two years of relevant experience in antibody purification is required
Demonstrable experience in implementing and conducting relevant experimental assays in a high-throughput manner. These assays include but are not limited to immunoassays, recombinant protein expression, protein purification, and industry-level quality control analysis and data reporting.
Proven track record for performing molecular biology experiments, including PCR, cloning and strain maintenance.
Experience with automated instruments (i.e. liquid handlers, plate washers, and plate readers) in order to manage the high-throughput nature of this project.
Experience with phage display technology is required and a strong knowledge of molecular and cell-based technologies is expected.
Experience with expi293 production scales from ml to L scales
Experience with LIMS data entry and Ab development pipeline coordination
Experience in RG2-level tissue culture
Must be a solid team player with excellent interpersonal Skills
Exceptional problem solving and communication (oral and written) skills
Strong organizational, analytical and record-keeping skills
Proficiency with Microsoft Office Suite applications (e.g. PowerPoint, Excel, Word, etc.)
Sound knowledge of molecular and cellular biology techniques, specifically phage display technology.
Transient transfection with pSCTa vector of expi293 cells for expression of antibodies and antigens
Skilled in the following processes and techniques: Affinity purification of antibodies with Protein A resin, endotoxin testing and removal from antibody preparations, antibody staining of cell lines of interest for flow cytometry, phage techniques: infection, amplification, concentration, competitive Ab ELISA and phage ELISA strategies, High-density experience: 384 well format, multichannel experience for ELISA assays, Molecular techniques : PCR, enzymatic digests, cloning, sequence analysis: Geneious software, protein characterization: SDS-PAGE analysis, Western Analysis, FPLC analysis (SEC), and thermal shift assays

Assets (Nonessential):
A background in molecular oncology, immunology or cellular biology

To be successful in this role you will be:
Team player

Travel: None

Notes: This is a 1 year term position